I'm very sad to see my thread for this topic didn't make the leap. I had linked some great discussions.
Director Marc Webb (ha!) previously of "(500) Days of Summer" fame (his only really big motion picture directing gig might I add), and once well known director of many alternative rock band videos for bands such as 3 Doors Down, Green Day, and I think one or two blink-182 songs, has stated repeatedly that this film is about a "back to basics" approach with Spider-Man.
They've completely withdrawn the whole Osborn/Parker conflict, Harry is supposedly in the film but how much of his father we'll see is unknown. The Staceys are in this film, and Gwen is the love interest (as it should be, honestly, in the beginning of Peter's story). His parents are tragically killed in a mysterious plane accident, he is adopted by his uncle Ben and aunt May, and Ben even has a severely relevant role in the film. According to most reports from producers such as Avi Arad, Ben won't meet his end for quite some time in the movie. This really is an ORIGIN story, not just a "first film". That's what I'm most excited about.
3-D I could live without, but you know... if there's one character 3-D is meant for, it's probably the Wallcrawler.